Small Fragment Homologous Replacement: Evaluation of Factors Influencing Modification Efficiency in an Eukaryotic Assay System

Homologous Replacement is used to modify specific gene sequences of chromosomal DNA in a process referred to as “Small Fragment Homologous Replacement”, where DNA fragments replace genomic target resulting in specific sequence changes. To optimize the efficiency of this process, we developed a repor...

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Hlavní autoři: Luchetti, Andrea, Filareto, Antonio, Sanchez, Massimo, Ferraguti, Giampiero, Lucarelli, Marco, Novelli, Giuseppe, Sangiuolo, Federica, Malgieri, Arianna
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science 2012
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Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3281040/
https://ncbi.nlm.nih.gov/pubmed/22359552
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0030851
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