Novel application of Locked Nucleic Acid chemistry for a TaqMan assay for measuring diverse Human Immunodeficiency Virus type 1 subtypes

There remains a need for sensitive and cost-effective assays to monitor therapy in human immunodeficiency virus type-1 (HIV-1) infection. However, the genetic diversity of HIV poses difficulties for traditional real-time PCR assays that require long oligonucleotides probes. LNA(™) probes may be usef...

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Detalhes bibliográficos
Main Authors: Li, Peilin, Ruel, Theodore, Fujimoto, Katsuya, Hatano, Hiroyu, Yukl, Steven, Eller, Leigh Anne, Liegler, Teri, Kamya, Moses, Gassasira, Anne, Dorsey, Grant, Rosenthal, Phil, Havlir, Diane, Wong, Joseph K.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3280821/
https://ncbi.nlm.nih.gov/pubmed/20863855
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2010.09.011
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