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Leu-enkephalin purification from E. coli cells carrying the plasmid with fused synthetic leu-enkephalin gene
Chemically synthesized leu-enkephalin gene was fused to a large Eco RI-Bam HI fragment of pBR322 along with a Eco RI fragment of Ch4A phage DNA carrying the promoter and most of the E.coli β-galactosidase gene. The resulting recombinant DNA was used to transform E. coli cells. Transformants were scr...
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| Main Authors: | , , , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1980
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC328079/ https://ncbi.nlm.nih.gov/pubmed/7008033 |
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