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Leu-enkephalin purification from E. coli cells carrying the plasmid with fused synthetic leu-enkephalin gene

Chemically synthesized leu-enkephalin gene was fused to a large Eco RI-Bam HI fragment of pBR322 along with a Eco RI fragment of Ch4A phage DNA carrying the promoter and most of the E.coli β-galactosidase gene. The resulting recombinant DNA was used to transform E. coli cells. Transformants were scr...

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Autors principals: Shemyakin, M.F., Chestukhin, A.V., Dolganov, G.M., Khodkova, E.M., Monastyrskaya, G.S., Sverdlov, E.D.
Format: Artigo
Idioma:Inglês
Publicat: 1980
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC328079/
https://ncbi.nlm.nih.gov/pubmed/7008033
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