Leu-enkephalin purification from E. coli cells carrying the plasmid with fused synthetic leu-enkephalin gene

Chemically synthesized leu-enkephalin gene was fused to a large Eco RI-Bam HI fragment of pBR322 along with a Eco RI fragment of Ch4A phage DNA carrying the promoter and most of the E.coli β-galactosidase gene. The resulting recombinant DNA was used to transform E. coli cells. Transformants were scr...

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Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	Shemyakin, M.F., Chestukhin, A.V., Dolganov, G.M., Khodkova, E.M., Monastyrskaya, G.S., Sverdlov, E.D.
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 1980
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC328079/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7008033/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/8.24.6163
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