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Production of specific site probes of tRNA structure by enrichment with carbon 13 at particular locations.
Escherichia coli C6 rel met cys was cultured in a stringently defined minimal medium containing 13C-enriched metabolites in order to (1) achieve maximal 13C isotopic enrichment of tRNA; and (2) produce site specific but natural, non-perturbing NMR probes of tRNA structure and function. Growth condit...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1979
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC328054/ https://ncbi.nlm.nih.gov/pubmed/388347 |
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