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Rapid synthesis of oligodeoxyribonucleotides. III. Effect of added carboxylate ion on the efficiency of internucleotide bond formation. Solid-phase synthesis of the dodecanucleotide, d(pT-A-A-C-T-G-C-T-C-A-C-T).

In solid-phase oligodeoxyribonucleotide synthesis the addition of benzoic acid to internucleotide phosphodiester bond-forming reactions lowers coupling yields by far greater amount than anticipated on a simple nucleotide-carboxylate molar ratio. High coupling yields were consistently obtained in syn...

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Bibliographic Details
Main Authors: Gait, M J, Sheppard, R C
Format: Artigo
Language:Inglês
Published: 1979
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC327771/
https://ncbi.nlm.nih.gov/pubmed/450696
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