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Addition of oligonucleotides to the 5'-terminus of DNA by T4 RNA ligase.
Bacteriophage T4-induced RNA ligase catalyzes the controlled template-independent addition of RNA to the 5'-phosphoryl end of large DNA molecules. Restriction enzyme-generated fragments of Co1E1 DNA with completely basepaired or cohesive ends and linear single-stranded øX174 viral DNA were all...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1979
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC327749/ https://ncbi.nlm.nih.gov/pubmed/375192 |
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