Addition of oligonucleotides to the 5'-terminus of DNA by T4 RNA ligase.
Bacteriophage T4-induced RNA ligase catalyzes the controlled template-independent addition of RNA to the 5'-phosphoryl end of large DNA molecules. Restriction enzyme-generated fragments of Co1E1 DNA with completely basepaired or cohesive ends and linear single-stranded øX174 viral DNA were all good...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1979
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327749/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/375192/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/6.3.1013 |
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