A new method for 3'-labelling of polyribonucleotides by phosphorylation with RNA ligase and its application to the 3'-modification for joining reactions.

P1-Adenosine 5'-P2-o-nitrobenzyl pyrophosphate (nbzlppA) has been synthesized as a substrate for T4 RNA ligase catalyzed 3'-phosphorylation. Incubation of oligoribonucleotides and nbzlppA with RNA ligase yielded oligoribonucleotides having a 3'-o-(o-nitrobenzyl) phosphate. Photochemic...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Ohtsuka, E, Uemura, H, Doi, T, Miyake, T, Nishikawa, S, Ikehara, M
Formato: Artigo
Idioma:Inglês
Publicado em: 1979
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC327706/
https://ncbi.nlm.nih.gov/pubmed/424301
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados