Elongation of oligonucleotides in the 3'-direction with activated mononucleotides and their analogs using RNA ligase.
P1-Adenosine 5'-P2-2',3'-ethoxymethylidene nucleosides [A(5')ppN(Em)] from four common nucleosides have been prepared and used for single addition of nucleotides to elongate oligonucleotide chains in the 3'-direction in RNA ligase reaction. U-U-C, T-U-C and A-C-C were used as acceptors. Structural d...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1980
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327293/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7443536/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/8.3.601 |
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