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Elongation of oligonucleotides in the 3'-direction with activated mononucleotides and their analogs using RNA ligase.

P1-Adenosine 5'-P2-2',3'-ethoxymethylidene nucleosides [A(5')ppN(Em)] from four common nucleosides have been prepared and used for single addition of nucleotides to elongate oligonucleotide chains in the 3'-direction in RNA ligase reaction. U-U-C, T-U-C and A-C-C were used as acceptors. Structural d...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Principais autores: Ohtsuka, E, Miyake, T, Nagao, K, Uemura, H, Nishikawa, S, Sugiura, M, Ikehara, M
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1980
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327293/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7443536/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/8.3.601
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