A new method for 3'-labelling of polyribonucleotides by phosphorylation with RNA ligase and its application to the 3'-modification for joining reactions.
P1-Adenosine 5'-P2-o-nitrobenzyl pyrophosphate (nbzlppA) has been synthesized as a substrate for T4 RNA ligase catalyzed 3'-phosphorylation. Incubation of oligoribonucleotides and nbzlppA with RNA ligase yielded oligoribonucleotides having a 3'-o-(o-nitrobenzyl) phosphate. Photochemical removal of t...
Sparad:
| I publikationen: | Nucleic Acids Res |
|---|---|
| Huvudupphov: | , , , , , |
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Utgiven: |
Oxford University Press
1979
|
| Länkar: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327706/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/424301/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/6.2.443 |
| Taggar: |
Inga taggar, Lägg till första taggen!
|