A new method for 3'-labelling of polyribonucleotides by phosphorylation with RNA ligase and its application to the 3'-modification for joining reactions.

P1-Adenosine 5'-P2-o-nitrobenzyl pyrophosphate (nbzlppA) has been synthesized as a substrate for T4 RNA ligase catalyzed 3'-phosphorylation. Incubation of oligoribonucleotides and nbzlppA with RNA ligase yielded oligoribonucleotides having a 3'-o-(o-nitrobenzyl) phosphate. Photochemic...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Main Authors: Ohtsuka, E, Uemura, H, Doi, T, Miyake, T, Nishikawa, S, Ikehara, M
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1979
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327706/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/424301/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/6.2.443
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