Highly Parallel Genome-Wide Expression Analysis of Single Mammalian Cells

BACKGROUND: We have developed a high-throughput amplification method for generating robust gene expression profiles using single cell or low RNA inputs. METHODOLOGY/PRINCIPAL FINDINGS: The method uses tagged priming and template-switching, resulting in the incorporation of universal PCR priming site...

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Detalles Bibliográficos
Main Authors: Fan, Jian-Bing, Chen, Jing, April, Craig S., Fisher, Jeffrey S., Klotzle, Brandy, Bibikova, Marina, Kaper, Fiona, Ronaghi, Mostafa, Linnarsson, Sten, Ota, Takayo, Chien, Jeremy, Laurent, Louise C., Nisperos, Sean V., Chen, Gina Y., Zhong, Jiang F.
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science 2012
Assuntos:
Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3275609/
https://ncbi.nlm.nih.gov/pubmed/22347404
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0030794
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