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Changes in Chromatin Compaction During the Cell Cycle Revealed by Micrometer-Scale Measurement of Molecular Flow in the Nucleus

We present a quantitative fluctuation-based assay to measure the degree of local chromatin compaction and investigate how chromatin density regulates the diffusive path adopted by an inert protein in dividing cells. The assay uses CHO-K1 cells coexpressing untagged enhanced green fluorescent protein...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Hinde, Elizabeth, Cardarelli, Francesco, Digman, Michelle A., Gratton, Enrico
Format: Artigo
Sprache:Inglês
Veröffentlicht: The Biophysical Society 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3274830/
https://ncbi.nlm.nih.gov/pubmed/22325293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2011.11.4026
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