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Changes in Chromatin Compaction During the Cell Cycle Revealed by Micrometer-Scale Measurement of Molecular Flow in the Nucleus

We present a quantitative fluctuation-based assay to measure the degree of local chromatin compaction and investigate how chromatin density regulates the diffusive path adopted by an inert protein in dividing cells. The assay uses CHO-K1 cells coexpressing untagged enhanced green fluorescent protein...

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Autors principals: Hinde, Elizabeth, Cardarelli, Francesco, Digman, Michelle A., Gratton, Enrico
Format: Artigo
Idioma:Inglês
Publicat: The Biophysical Society 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3274830/
https://ncbi.nlm.nih.gov/pubmed/22325293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2011.11.4026
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