A Rapid Subtractive Immunization Method to Prepare Discriminatory Monoclonal Antibodies for Food E. coli O157:H7 Contamination

To detect food E. coli O157:H7 contamination rapidly and accurately, it is essential to prepare high specific monoclonal antibodies (mAbs) against the pathogen. Cyclophosphamide (Cy)-mediated subtractive immunization strategy was performed in mice to generate mAbs that react with E. coli O157:H7, bu...

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Detalhes bibliográficos
Main Authors: Jin, Min, Lang, Jing, Shen, Zhi-Qiang, Chen, Zhao-Li, Qiu, Zhi-Gang, Wang, Xin-Wei, Li, Jun-Wen
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2012
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3274518/
https://ncbi.nlm.nih.gov/pubmed/22347466
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0031352
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