Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract

We describe the preparation of a cell-free extract from Drosophila Kc cells which allows transcription of a variety of cloned eukaryotic RNA polymerase III genes. The extract has low RNA-processing nuclease activity and thus the major products obtained are primary transcripts.

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Bibliografiske detaljer
Main Authors: Dingermann, Theodor, Sharp, Stephen, Appel, Bernd, DeFranco, Donald, Mount, Stephen, Heiermann, Reinhard, Pongs, Olaf, Söll, Dieter
Format: Artigo
Sprog:Inglês
Udgivet: 1981
Fag:
Molecular Biology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC327404/
https://ncbi.nlm.nih.gov/pubmed/6170932
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