Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract

We describe the preparation of a cell-free extract from Drosophila Kc cells which allows transcription of a variety of cloned eukaryotic RNA polymerase III genes. The extract has low RNA-processing nuclease activity and thus the major products obtained are primary transcripts. IMAGES:

Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	Dingermann, Theodor, Sharp, Stephen, Appel, Bernd, DeFranco, Donald, Mount, Stephen, Heiermann, Reinhard, Pongs, Olaf, Söll, Dieter
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 1981
Fag:	Molecular Biology
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327404/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6170932/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/9.16.3907
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