Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract
We describe the preparation of a cell-free extract from Drosophila Kc cells which allows transcription of a variety of cloned eukaryotic RNA polymerase III genes. The extract has low RNA-processing nuclease activity and thus the major products obtained are primary transcripts. IMAGES:
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| Yayımlandı: | Nucleic Acids Res |
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| Asıl Yazarlar: | , , , , , , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Oxford University Press
1981
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327404/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6170932/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/9.16.3907 |
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