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Oligonucleotide directed mutagenesis of the human beta-globin gene: a general method for producing specific point mutations in cloned DNA.

A nonadecanucleotide has been used both as a site specific mutagen to introduce a T leads to A transversion mutation in the human beta-globin gene cloned in pBR322 as well as a probe to screen transformed colonies for the desired mutant. The specificity of the oligonucleotide as a mutagen and as a h...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Wallace, R B, Schold, M, Johnson, M J, Dembek, P, Itakura, K
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 1981
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC327381/
https://ncbi.nlm.nih.gov/pubmed/7279669
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