Physical and kinetic properties of the site specific endonuclease Bam HI from Bacillus amylolique-faciens.
The site specific endonuclease Bam HI which is composed of subunits of a molecular weight of 22 000 [1] can aggregate to complexes of a molecular weight of 360 000. It is an acidic protein with an isoelectric point at pH 5.3. Optimal activity is reached at 13 mM MgCl2. A very simple method is presen...
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| Veröffentlicht in: | Nucleic Acids Res |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1980
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327295/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6255448/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/8.3.623 |
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