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Physical and kinetic properties of the site specific endonuclease Bam HI from Bacillus amylolique-faciens.
The site specific endonuclease Bam HI which is composed of subunits of a molecular weight of 22 000 [1] can aggregate to complexes of a molecular weight of 360 000. It is an acidic protein with an isoelectric point at pH 5.3. Optimal activity is reached at 13 mM MgCl2. A very simple method is presen...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1980
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC327295/ https://ncbi.nlm.nih.gov/pubmed/6255448 |
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