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Elongation of oligonucleotides in the 3'-direction with activated mononucleotides and their analogs using RNA ligase.

P1-Adenosine 5'-P2-2',3'-ethoxymethylidene nucleosides [A(5')ppN(Em)] from four common nucleosides have been prepared and used for single addition of nucleotides to elongate oligonucleotide chains in the 3'-direction in RNA ligase reaction. U-U-C, T-U-C and A-C-C were used a...

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Main Authors: Ohtsuka, E, Miyake, T, Nagao, K, Uemura, H, Nishikawa, S, Sugiura, M, Ikehara, M
Formato: Artigo
Idioma:Inglês
Publicado: 1980
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC327293/
https://ncbi.nlm.nih.gov/pubmed/7443536
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