Joining of ribooligonucleotides with T4 RNA ligase and identification of the oligonucleotide-adenylate intermediate.
T4 RNA ligase was found to join A-A-A-A-A-A and 32pU-U-U-U in the presence of ATP as cofactor. In this reaction the pyrophosphate of pU-U-U-U and pA was isolated by chromatography on a RPC-5 column, besides the joined product and the starting materials. This pyrophosphate was shown to be an intermed...
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| Published in: | Nucleic Acids Res |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
1976
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| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC343018/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/183186/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/3.6.1613 |
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