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TROSY of side-chain amides in large proteins
By using the mixed solvent of 50% H(2)O/50% D(2)O and employing deuterium decoupling, TROSY experiments exclusively detect NMR signals from semideuterated isotopomers of carboxamide groups with high sensitivities for proteins with molecular weights up to 80 kDa. This isotopomer-selective strategy ex...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2007
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3272764/ https://ncbi.nlm.nih.gov/pubmed/17347000 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmr.2007.02.008 |
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