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Hydrogen-Bond Detection, Configuration Assignment, and Rotamer Correction of Side-Chain Amides in Large Proteins by NMR through Protium/Deuterium Isotope Effects
In this study, the configuration and hydrogen-bonding network of side-chain amides in a 35 kDa protein are determined via measuring differential and across-hydrogen-bond H/D isotope effects by the IS-TROSY technique, which leads to a reliable recognition and correction of erroneous rotamers frequent...
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| Autors principals: | , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2008
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3018730/ https://ncbi.nlm.nih.gov/pubmed/18973166 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.200800467 |
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