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Characterization of a R plasmid-associated, trimethoprim-resistant dihydrofolate reductase and determination of the nucleotide sequence of the reductase gene.
The trimethoprim-resistant dihydrofolate reductase associated with the R plasmid R388 was isolated from strains that over-produce the enzyme. It was purified to apparent homogeneity by affinity chromatography and two consecutive gel filtration steps under native and denaturing conditions. The purifi...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1981
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC327231/ https://ncbi.nlm.nih.gov/pubmed/6261228 |
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