m-Aminophenylboronate agarose specifically binds capped snRNA and mRNA.

m-Aminophenylboronate-substituted agarose binds specifically RNA chains carrying a mature 5' cap. The binding occurs most effectively at pH greater than 8 and involves diester formation between the negatively charged tetrahedric boronate group and the cisdiol of the ribose of the cap. The posit...

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Bibliographic Details
Main Authors: Wilk, H E, Kecskemethy, N, Schäfer, K P
Format: Artigo
Language:Inglês
Published: 1982
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC327034/
https://ncbi.nlm.nih.gov/pubmed/6185925
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