m-Aminophenylboronate agarose specifically binds capped snRNA and mRNA.
m-Aminophenylboronate-substituted agarose binds specifically RNA chains carrying a mature 5' cap. The binding occurs most effectively at pH greater than 8 and involves diester formation between the negatively charged tetrahedric boronate group and the cisdiol of the ribose of the cap. The positive c...
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| Publicado en: | Nucleic Acids Res |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
1982
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327034/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6185925/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.23.7621 |
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