m-Aminophenylboronate agarose specifically binds capped snRNA and mRNA.

m-Aminophenylboronate-substituted agarose binds specifically RNA chains carrying a mature 5' cap. The binding occurs most effectively at pH greater than 8 and involves diester formation between the negatively charged tetrahedric boronate group and the cisdiol of the ribose of the cap. The positive c...

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izdano v:	Nucleic Acids Res
Principais autores:	Wilk, H E, Kecskemethy, N, Schäfer, K P
Format:	Artigo
Jezik:	Inglês
Izdano:	Oxford University Press 1982
Teme:	Research Article
Online dostop:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327034/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6185925/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.23.7621
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