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Proximity of the invariant loop of U5 snRNA to the second intron residue during pre-mRNA splicing

A photoactivatable azidophenacyl group has been introduced into seven positions in the backbone of the 11 nucleotide invariant loop of U5 snRNA. By reconstituting depleted splicing extracts with reassembled U5 snRNP particles, molecular neighbors were assessed as a function of splicing. All cross-li...

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Autores principales: McConnell, Timothy S., Steitz, Joan A.
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2001
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC125517/
https://ncbi.nlm.nih.gov/pubmed/11432844
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/20.13.3577
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