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Proximity of the invariant loop of U5 snRNA to the second intron residue during pre-mRNA splicing

A photoactivatable azidophenacyl group has been introduced into seven positions in the backbone of the 11 nucleotide invariant loop of U5 snRNA. By reconstituting depleted splicing extracts with reassembled U5 snRNP particles, molecular neighbors were assessed as a function of splicing. All cross-li...

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Bibliografiske detaljer
Main Authors: McConnell, Timothy S., Steitz, Joan A.
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2001
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC125517/
https://ncbi.nlm.nih.gov/pubmed/11432844
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/20.13.3577
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