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Proximity of the invariant loop of U5 snRNA to the second intron residue during pre-mRNA splicing

A photoactivatable azidophenacyl group has been introduced into seven positions in the backbone of the 11 nucleotide invariant loop of U5 snRNA. By reconstituting depleted splicing extracts with reassembled U5 snRNP particles, molecular neighbors were assessed as a function of splicing. All cross-li...

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Détails bibliographiques
Publié dans:EMBO J
Auteurs principaux: McConnell, Timothy S., Steitz, Joan A.
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group 2001
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC125517/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11432844/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/emboj/20.13.3577
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