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Simple, efficient in vitro synthesis of capped RNA useful for direct expression of cloned eukaryotic genes.

A simple and efficient method for direct in vitro synthesis of capped transcripts of cloned eukaryotic genes is described. As an example capped transcripts were made from a plasmid containing the human fibroblast interferon gene cloned under the control of a prokaryotic promoter. These transcripts w...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Principais autores: Contreras, R, Cheroutre, H, Degrave, W, Fiers, W
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326924/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6294601/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.20.6353
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