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Simple, efficient in vitro synthesis of capped RNA useful for direct expression of cloned eukaryotic genes.

A simple and efficient method for direct in vitro synthesis of capped transcripts of cloned eukaryotic genes is described. As an example capped transcripts were made from a plasmid containing the human fibroblast interferon gene cloned under the control of a prokaryotic promoter. These transcripts w...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Contreras, R, Cheroutre, H, Degrave, W, Fiers, W
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1982
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC326924/
https://ncbi.nlm.nih.gov/pubmed/6294601
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