Simple, efficient in vitro synthesis of capped RNA useful for direct expression of cloned eukaryotic genes.

A simple and efficient method for direct in vitro synthesis of capped transcripts of cloned eukaryotic genes is described. As an example capped transcripts were made from a plasmid containing the human fibroblast interferon gene cloned under the control of a prokaryotic promoter. These transcripts w...

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Bibliografische gegevens

Gepubliceerd in:	Nucleic Acids Res
Hoofdauteurs:	Contreras, R, Cheroutre, H, Degrave, W, Fiers, W
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	Oxford University Press 1982
Onderwerpen:	Research Article
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326924/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6294601/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/10.20.6353
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