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Uracil DNa-glycosylase from HeLa cells: general properties, substrate specificity and effect of uracil analogs.

Uracil-DNA glycosylase was partially purified from HeLa cells. Various substrates containing [3H]dUMP residues were prepared by nick-translation of calf thymus DNA. The standard substrate was double-stranded DNA with [3H]dUMP located internally in the chain. Compared to the release of uracil from th...

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Publicat a:Nucleic Acids Res
Autors principals: Krokan, H, Wittwer, C U
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1981
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326875/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7279657/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/9.11.2599
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