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Uracil DNa-glycosylase from HeLa cells: general properties, substrate specificity and effect of uracil analogs.
Uracil-DNA glycosylase was partially purified from HeLa cells. Various substrates containing [3H]dUMP residues were prepared by nick-translation of calf thymus DNA. The standard substrate was double-stranded DNA with [3H]dUMP located internally in the chain. Compared to the release of uracil from th...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1981
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC326875/ https://ncbi.nlm.nih.gov/pubmed/7279657 |
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