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Detection of the catalytic activities of DNA polymerases and their associated exonucleases following SDS-polyacrylamide gel electrophoresis.

A method is described to detect DNA polymerases and nucleases in homogeneous or crude enzyme preparations after electrophoresis in SDS-polyacrylamide gels(2) containing the appropriate template or substrate. DNA polymerases are electrophoresed in a gel containing gapped calf thymus DNA and after a r...

詳細記述

保存先:
書誌詳細
主要な著者: Spanos, A, Sedgwick, S G, Yarranton, G T, Hübscher, U, Banks, G R
フォーマット: Artigo
言語:Inglês
出版事項: 1981
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC326806/
https://ncbi.nlm.nih.gov/pubmed/6264395
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