Detection of the catalytic activities of DNA polymerases and their associated exonucleases following SDS-polyacrylamide gel electrophoresis.

A method is described to detect DNA polymerases and nucleases in homogeneous or crude enzyme preparations after electrophoresis in SDS-polyacrylamide gels(2) containing the appropriate template or substrate. DNA polymerases are electrophoresed in a gel containing gapped calf thymus DNA and after a r...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Nucleic Acids Res
Prif Awduron: Spanos, A, Sedgwick, S G, Yarranton, G T, Hübscher, U, Banks, G R
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 1981
Mynediad Ar-lein:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326806/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6264395/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/9.8.1825
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