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Detection of the catalytic activities of DNA polymerases and their associated exonucleases following SDS-polyacrylamide gel electrophoresis.

A method is described to detect DNA polymerases and nucleases in homogeneous or crude enzyme preparations after electrophoresis in SDS-polyacrylamide gels(2) containing the appropriate template or substrate. DNA polymerases are electrophoresed in a gel containing gapped calf thymus DNA and after a r...

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Bibliografske podrobnosti
Main Authors: Spanos, A, Sedgwick, S G, Yarranton, G T, Hübscher, U, Banks, G R
Format: Artigo
Jezik:Inglês
Izdano: 1981
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC326806/
https://ncbi.nlm.nih.gov/pubmed/6264395
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