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Molecular cloning of Chinese hamster dihydrofolate reductase-specific cDNA and the identification of multiple dihydrofolate reductase mRNAs in antifolate-resistant Chinese hamster lung fibroblasts.

ds cDNA from antifolate-resistant Chinese hamster lung fibroblast subline DC-3F/MQ19 was ligated to Eco RI and Sal I oligonucleotide linkers and cloned into Eco RI and Sal I digested pBR322. Transformed colonies containing dihydrofolate reductase (DHFR)-specific recombinant plasmid were identified b...

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Bibliografski detalji
Glavni autori: Lewis, J A, Kurtz, D T, Melera, P W
Format: Artigo
Jezik:Inglês
Izdano: 1981
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC326759/
https://ncbi.nlm.nih.gov/pubmed/6262725
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