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Selection and analysis of cloned developmentally-regulated Dictyostelium discoideum genes by hybridization-competition.
We describe a new technique for selection of cloned gene segments which are expressed preferentially at one developmental stage but at a relatively low level. A nitrocellulose filter replica of plaques of lambda phage which contain approximately 8 KB inserts of genomic DNA is prepared; it is hybridi...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1981
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC326724/ https://ncbi.nlm.nih.gov/pubmed/7232208 |
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