Selection and analysis of cloned developmentally-regulated Dictyostelium discoideum genes by hybridization-competition.
We describe a new technique for selection of cloned gene segments which are expressed preferentially at one developmental stage but at a relatively low level. A nitrocellulose filter replica of plaques of lambda phage which contain approximately 8 KB inserts of genomic DNA is prepared; it is hybridi...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1981
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326724/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7232208/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/9.4.947 |
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