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Selection and analysis of cloned developmentally-regulated Dictyostelium discoideum genes by hybridization-competition.

We describe a new technique for selection of cloned gene segments which are expressed preferentially at one developmental stage but at a relatively low level. A nitrocellulose filter replica of plaques of lambda phage which contain approximately 8 KB inserts of genomic DNA is prepared; it is hybridi...

詳細記述

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書誌詳細
主要な著者: Mangiarotti, G, Chung, S, Zuker, C, Lodish, H F
フォーマット: Artigo
言語:Inglês
出版事項: 1981
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC326724/
https://ncbi.nlm.nih.gov/pubmed/7232208
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