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Temperature dependence of the joining by T4 DNA ligase of termini produced by type II restriction endonucleases.

The temperature dependence of the T4 DNA ligase-catalyzed joining of plasmid DNA linearized by the action of HaeIII, EcoRI and PstI restriction endonucleases has been investigated by electron microscopy analysis. The extent of joining is maximal at 4 degrees and decreases with increasing temperature...

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Bibliografiska uppgifter
Huvudupphovsmän: Ferretti, L, Sgaramella, V
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1981
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC326670/
https://ncbi.nlm.nih.gov/pubmed/6259621
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