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GPCR stabilization using the bicelle-like architecture of mixed sterol-detergent micelles

The biophysical characterization of purified membrane proteins typically requires detergent mediated extraction from native lipid membrane environments. In the case of human G protein-coupled receptors (GPCRs), this process has been complicated by their conformational heterogeneity and the general l...

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Autori principali: Thompson, Aaron A., Liu, Jeffrey J., Chun, Eugene, Wacker, Daniel, Wu, Huixian, Cherezov, Vadim, Stevens, Raymond C.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2011
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3264755/
https://ncbi.nlm.nih.gov/pubmed/22041719
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2011.10.011
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