Chemical synthesis of a human interferon-alpha 2 gene and its expression in Escherichia coli.

A 511-base pair DNA fragment encoding human interferon-alpha 2 has been chemically synthesised and expressed from a lac UV5 and a synthetic trp promoter in Escherichia coli. The synthesis involved preparation of 68 oligodeoxyribonucleotides and their enzymic ligation. The product expressed from the...

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Detaylı Bibliyografya
Yayımlandı:Nucleic Acids Res
Asıl Yazarlar: Edge, M D, Greene, A R, Heathcliffe, G R, Moore, V E, Faulkner, N J, Camble, R, Petter, N N, Trueman, P, Schuch, W, Hennam, J
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Oxford University Press 1983
Online Erişim:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326383/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6312423/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.18.6419
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