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Chemical synthesis of a human interferon-alpha 2 gene and its expression in Escherichia coli.
A 511-base pair DNA fragment encoding human interferon-alpha 2 has been chemically synthesised and expressed from a lac UV5 and a synthetic trp promoter in Escherichia coli. The synthesis involved preparation of 68 oligodeoxyribonucleotides and their enzymic ligation. The product expressed from the...
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| Hauptverfasser: | , , , , , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1983
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC326383/ https://ncbi.nlm.nih.gov/pubmed/6312423 |
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