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Chemical synthesis of a human interferon-alpha 2 gene and its expression in Escherichia coli.
A 511-base pair DNA fragment encoding human interferon-alpha 2 has been chemically synthesised and expressed from a lac UV5 and a synthetic trp promoter in Escherichia coli. The synthesis involved preparation of 68 oligodeoxyribonucleotides and their enzymic ligation. The product expressed from the...
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| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , , , , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1983
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| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC326383/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6312423/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/11.18.6419 |
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