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Essential structure of E. coli promoter: effect of spacer length between the two consensus sequences on promoter function.

A promoter with the consensus sequence(TTGACA and TATAAT) at -35 and -10 regions was constructed, and the distance between the two consensus sequences was independently altered at two restriction sites. In an in vitro transcription system, a maximum activity was observed at the spacer length of 17 b...

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Autors principals: Aoyama, T, Takanami, M, Ohtsuka, E, Taniyama, Y, Marumoto, R, Sato, H, Ikehara, M
Format: Artigo
Idioma:Inglês
Publicat: 1983
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC326322/
https://ncbi.nlm.nih.gov/pubmed/6310517
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