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Essential structure of E. coli promoter: effect of spacer length between the two consensus sequences on promoter function.
A promoter with the consensus sequence(TTGACA and TATAAT) at -35 and -10 regions was constructed, and the distance between the two consensus sequences was independently altered at two restriction sites. In an in vitro transcription system, a maximum activity was observed at the spacer length of 17 b...
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| Main Authors: | , , , , , , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1983
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC326322/ https://ncbi.nlm.nih.gov/pubmed/6310517 |
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