Synthetic biology approach to reconstituting the ubiquitylation cascade in bacteria

Covalent modification of proteins with ubiquitin (Ub) is widely implicated in the control of protein function and fate. Over 100 deubiquitylating enzymes rapidly reverse this modification, posing challenges to the biochemical and biophysical characterization of ubiquitylated proteins. We circumvente...

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Main Authors: Keren-Kaplan, Tal, Attali, Ilan, Motamedchaboki, Khatereh, Davis, Brian A, Tanner, Neta, Reshef, Yael, Laudon, Einat, Kolot, Mikhail, Levin-Kravets, Olga, Kleifeld, Oded, Glickman, Michael, Horazdovsky, Bruce F, Wolf, Dieter A, Prag, Gali
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group 2012
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Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3261559/
https://ncbi.nlm.nih.gov/pubmed/22081111
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/emboj.2011.397
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